Active Recombinant Human FCGR1A, His-tagged

Specification :
| Description : Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. | Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. | Bio-activity : Scatchard analysis showed estimated the affinity constant ( KD ) of CD64 bound to Human IgG1 was less than 0.138 nM. | Molecular Mass : rh CD64 / FCGR1A is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 32.5 kDa. The predicted N-terminus is Gln 16 . DTT-reduced Protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation. | Endotoxin : Less than 1.0 EU per μg of the rh CD64 / FCGR1A by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC). | Reconstitution : See Certificate of Analysis for reconstitution instructions and specific concentrations.