Active Recombinant Human FCGR2A protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. | Bio-activity : Biotinylated Human CD32a (R167), His,Avitag captured on Biotin CAP-Series S Sensor Chip can bind Rituximab with an affinity constant of 2.53 μM as determined in a SPR assay (Biacore 8K).Biotinylated Human CD32a (R167), His,Avitag immobilized on SA Chip can bind Rituximab with an affinity constant of 0.802 μM as determined in a SPR assay (Biacore T200). | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 23.9 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Ala36-Ile218 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.