Active Recombinant Human FCGR2A, His & AVI Tagged, Biotinylated, 167 His

Specification :
| Description : Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94 – 99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fcγ RII A is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non- hematopoietic origin. Associated with an ITAM (immunoreceptor tyrosine-based activation motif)- bearing adapter subunit, Fc Rγ, CD32a (Fcγ RII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. | Source : Human Cells | Species : Human | Tag : His & AVI Tag | Form : Lyophilized from sterile PBS , pH 7.4 | Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. | Molecular Mass : The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions. | Protein length : Met1-Met210 | Endotoxin : | Purity : >95 % as determined by SDS-PAGE | Storage : Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : Reconstitute with water to desired concentration.