Active Recombinant Human FCGR2A Protein, His-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His | Predicted N Terminal : Ala 36 | Form : Lyophilized from sterile PBS, pH7.4, 10% Trehalose. | Bio-activity : The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay. Immobilized Human FcGR2A / CD32a (H131), His Tag can bind Ipilimumab with affinity constant of around 2.3 uM. Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (0.5 ug/well). The linear range is 0.5-16 ng/mL. | Molecular Mass : This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24 kDa. The protein migrates as 30-38 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.