Active Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His & Avi | Form : Lyophilized from sterile PBS, pH 7.4Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : 1. Measured by its ability to bind human IgG1 in functional ELISA.2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated bound to Avastin was 0.02 μM. | Molecular Mass : The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation. | Protein length : Ala 46-Ile 224 | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.