Active Recombinant Human FCGR3A(F176) protein, His/Avi-tagged, Biotinylated

Specification :
| Description : FCGR3A is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptorexpressed by NK cells, T cells, monocytes, and macrophages (1). Fcγ RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain(ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fcγ RIIIa shares 63%, 61%, 65%, 59% and 58% aa identitywith mouse Fcγ RIV, rat Fcγ RIIIa, feline CD16, bovine CD16 and porcine Fcγ RIIIb paralogs, respectively. The Fcγ RIIIa cDNA encodes 254 aa including a 16 aasignal sequence, 192 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 21 aa transmembrane (TM) sequence and a 25 aacytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influencesusceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # AVI8894 is expressed as the F176 isoform of Fcγ RIIIa. Fcγ RIIIasurface expression requires interaction of an accessory chain, either the common γ-chain or CD3ζ (5, 6). Glycosylation patterns, electrophoretic mobility and bindingaffinity appear to differ between NK cell and monocyte Fcγ RIIIa (7). The ECD of both Fcγ RIIIa and b can be proteolytically cleaved and retain binding activity insoluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trIgGer Fcγ RIIIa release (11). Soluble Fcγ RIII can be detected in normalplasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10). | Source : HEK293 cells | Species : Human | Tag : His/Avi | Predicted N Terminal : Gly17 | Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | Bio-activity : The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human FCGR3A(F176) His-tag Avi-tag binds with an ED50 of 50-300 ng/mL. | Molecular Mass : 48-57 kDa, under reducing conditions | Protein length : Gly17-Gln208 | Endotoxin : | Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. | Applications : Bioactivity | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 200 μg/mL in PBS.