Active Recombinant Human FCGR3A protein(F176), His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. | Bio-activity : Loaded Biotinylated Human CD16a (F176), Avitag,His Tag on SA Biosensor, can bind Rituximab with an affinity constant of 1.29 μM as determined in BLI assay (ForteBio Octet Red96e). | Molecular Mass : This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Gly17-Gln208 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.