Active Recombinant Human FCGR3A protein, His/Avi-tagged, Biotinylated

Specification :
| Description : FCGR3A/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). In humans, it is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (4). The ECD of FCGR3A also shares 63%, 61%, 65%, 59%, and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIA, feline CD16, bovine CD16, and porcine Fc gamma RIIIB, respectively. Mature human Fc gamma RIIIA consists of a 192 aa ECD with two C2-type Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (5, 6). Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta (7, 8). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (9). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (10-13). Shedding from monocytes and macrophages can be trIgGered by cell activation or phagocytosis (13). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (11, 12). Our Avi-tag Biotinylated human Fc gamma RIIIA features biotinylation at a single site contained within the Avi-tag, a unique 15 aa peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein’s bioactivity. | Source : HEK293 cells | Species : Human | Tag : His/Avi | Predicted N Terminal : Gly17 | Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | Bio-activity : The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human FCGR3A His-tag Avi-tag with an ED50 of 4-40 ng/mL. | Molecular Mass : 48-57 kDa, under reducing conditions | Protein length : Gly17-Gln208 | Endotoxin : | Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. | Applications : Bioactivity | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 500 μg/mL in PBS.