Active Recombinant Human FCGR3A protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. | Bio-activity : 1.Immobilized Human FcγRIIIA / CD16a (V176) recombinant protein at 2 μg/mL can bind Human IgG1, the EC50 is 150-450 ng/mL.2.Loaded Human FcγRIIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay (ForteBio Octet Red384) .3.Captured Human FcγRIIIA / CD16a (V176) recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 μM as determined in an SPR assay (Biacore T200).4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein with an affinity constant of 0.78 µM as determined in an SPR assay (Biacore T200). | Molecular Mass : The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation. | Proteinlength : Met1-Gln208,F176V | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.