Recombinant Human Fibroblast Growth Factor Receptor 2, Fc Chimera

Specification :
| Cat. No. : FGFR2-595H | Description : Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally realted proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family if type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the á isoform, or only IgII and IgIII, referred to as the ß isoform. Only the á isoform has been identified for FGFR-3 and FGFR-4. | Source : E.coli. | Subunit : Glycosylated dimer. | Formulation : Lyophilized. | Endotoxin Level : | Purity : > 90%, by SDS-PAGE and visualised by silver stain. | Reconstitution : The lyophilised sFGFR-2á (IIIc)/Fc is soluble in water and most aqueous buffers. The lyophilised sFGF-R2á (IIIc)/Fc should be reconstituted in PBS or medium to a concentration not l lower than 50µg/ml. | Biological Activity : Determined by its ability to inhibit human FGF acidic-dependent proliferation on R1 cells. The ED50 for this effect is typically at 15.0-30.0 ng/ml. | Stability : Lyophilised samples are stable for greater than six months at –20°C to –70℃. Reconstituted sFGFR-2á (IIIc)/Fc should be stored in working aliquots at -20℃. Avoid repeated freeze-thaw cycles!