Active Recombinant Human FLT1 protein, Fc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : Fc | Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human VEGFR1-Fc at 10μg/mL (100μL/well) can bind biotinylated human VEGF165, the EC50 of biotinylated human VEGF165 is 10-40 ng/mL. 2. Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-100 ng/mL in the presence of 10 ng/mL rhVEGF165. | Molecular Mass : The recombinant human FLT1 consists 543 amino acids and predicts a molecular mass of 61.1 kDa. | Protein Length : Met1-Ile328 | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. | Storage : Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.