Active Recombinant Human FLT1 protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4 | Bio-activity : Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). (Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323.)The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165. | Molecular Mass : The recombinant human FLT1 consists 313 amino acids and predicts a molecular mass of 35.6 kDa. | Protein Length : Met1-Ile328 | Endotoxin : | Purity : > 90 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.