Recombinant human FLT3, Active

Specification :
| Description : FLt3 ligand is a transmembrane glycoprotein that activates the FLt3 receptor (CD135, FLK2). FLT3 ligand, in synergy with other cytokines, stimulates the growth and differentiation of a number of important cell populations. Activation of FLt3 stimulates the growth and differentiation of very early stem cells involved in haematopoiesis, myelopoiesis, erythropoiesis and megakaryopoiesis. FLt3 activation mobilizes hematopoietic progenitor cells into peripheral blood and induces proliferation of myeloid and dendritic cells (DC). Dysregulation of FLt3 has been associated with acute leukaemia(s) involving myeloid (AML), T, and B blast cell(s). | Source : Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product. | Species : Human | Form : Recombinant human Flt3 is lyophilized from a Tris HCl 0.05M buffer pH 7.4. | Molecular Mass : 18.4 kDa, apparent molecular mass of 18-25 kDa in SDS-PAGE. | AA Sequence : HHHHHHTQDCSFQHSPISSDFAVKI RELSDYLLQDYPVTVASNLQDEELC GGLWRLVLAQRWMERLKTVAGSKMQ GLLERVNTEIHFVTKCAFQPPPSCL RFVQTNISRLLQETSEQLVALKPWI TRQNFSRCLELQCQPDSSTLPPPWS PRPLEATAPTA | Endotoxin : | Purity : >97% by SDS-PAGE gel | Storage : This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended. | Reconstitution : Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Optimal concentration should be determined for specific application and cell lines.