Name :
Recombinant Human GAK protein, GST-tagged
Specification :
| Description : Cyclin-G-associated kinase (GAK) is a serine/threonine protein kinase and was originally identified as a protein that is associated with cyclin G. However, neither CDK5 nor GAK kinase activity is activated by cyclin G nor does GAK affect CDK5 kinase activity. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. | Source : Insect cells | Species : Human | Tag : GST | Form : 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. | Molecular Mass : ~68 kDa | Purity : >95% | Applications : Kinase Assay | Stability : 1 year at -80 centigrade from the date of shipment | Storage : Store at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. | Concentration : 0.1μg/μl
Gene Information :
| Gene Name : GAK cyclin G associated kinase [ Homo sapiens ] | Official Symbol : GAK | Synonyms : GAK; cyclin G associated kinase; cyclin-G-associated kinase; auxilin 2; DNAJC26; auxilin-2; DNAJ26; FLJ16629; FLJ40395; MGC99654; | Gene ID : 2580 | mRNA Refseq : NM_005255 | Protein Refseq : NP_005246 | MIM : 602052 | UniProt ID : O14976 | Chromosome Location : 4p16 | Pathway : Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem; | Function : ATP binding; cyclin binding; heat shock protein binding; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups;
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