Recombinant Human GCK Protein, GST-tagged

Specification :
| Description : Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq | Source : Wheat Germ | Species : Human | Tag : GST | Molecular Mass : 76.78 kDa | AA Sequence : MLDDRARMEAAKKEKVEQILAEFQL QEEDLKKVMRRMQKEMDRGLRLETH EEASVKMLPTYVRSTPEGSEVGDFL SLDLGGTNFRVMLVKVGEGEEGQWS VKTKHQMYSIPEDAMTGTAEMLFDY ISECISDFLDKHQMKHKKLPLGFTF SFPVRHEDIDKGILLNWTKGFKASG AEGNNVVGLLRDAIKRRGDFEMDVV AMVNDTVATMISCYYEDHQCEVGMI VGTGCNACYMEEMQNVELVEGDEGR MCVNTEWGAFGDSGELDEFLLEYDR LVDESSANPGQQLYEKLIGGKYMGE LVRLVLLRLVDENLLFHGEASEQLR TRGAFETRFVSQVESDTGDRKQIYN ILSTLGLRPSTTDCDIVRRACESVS TRAAHMCSAGLAGVINRMRESRSED VMRITVGVDGSVYKLHPSFKERFHA SVRRLTPSCEITFIESEEGSGRGAA LVSAVACKKACMLGQ | Applications : Enzyme-linked Immunoabsorbent AssayWestern Blot (Recombinant protein)Antibody ProductionProtein Array | Notes : Best use within three months from the date of receipt of this protein. | Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. | Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.