Active Recombinant Human GPC3 protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. | Bio-activity : Immobilized Human FGF basic, premium grade at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His,Avitag, premium grade with a linear range of 0.5-16 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 26.4 kDa respectively. The protein migrates as 32-35 kDa, 40 kDa and 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Gln25-His559 | Endotoxin : Less than 0.01 EU per μg by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.