Active Recombinant Human HHLA2 Protein, Fc-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : Fc-Avi | Predicted N Terminal : Ile 23 | Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose. | Bio-activity : Immobilized Human CD28H, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-H7, Fc Tag, Avi Tag with a linear range of 5-78 ug/mL. | Molecular Mass : The protein has a calculated MW of 65.9 kDa. The protein migrates as 80-90 kDa under reducing condition (SDS-PAGE) due to glycosylation. | Endotoxin : | Purity : >90% as determined by SDS-PAGE. | Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.45-1 as determined by the HABA assay. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.