Active Recombinant Human FGFR2 protein, His&GST-tagged

Specification :
| Source : Insect Cells | Species : Human | Tag : His&GST | Form : Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA. | Bio-activity : 1. The specific activity was determined to be 28 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/ml.3. Measured by its binding ability in a functional ELISA. Immobilized recombinant recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic with a linear range of 0.16-1.25 μg/ml. | Molecular Mass : The recombinant human FGFR2 (aa 400-821) /GST chimera consists of 659 amino acids and predictes a molecular mass of 75.7 kDa. It migrates as an approximately 68 KDa band in SDS-PAGE under reducing conditions. | Protein Length : Met400-Thr821 | Endotoxin : | Purity : > 90 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.