Recombinant Human, HSPE1 His-tagged

Specification :
| Cat. No. : HSPE1-3679H | Description : Heat shock 10kDa protein 1 (chaperonin 10) , also known as HSPE1, GroES or cpn10 , is a chaperonin which usually works in conjunction with GroEL.[1] In eukaryotes, it is very similar to Heat Shock Protein 10 or Hsp10. | Source : E. coli | Species : Human | Form : Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4 | Purity : >90% | Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. | Applications : ELISA; Western blotting | Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin | Sequences of amino acids : MKHHHHHHAS AGQAFRKFLP LFDRVLVERS AAETVTKGGI MLPEKSQGKV LQATVVAVGS GSKGKGGEIQ PVSVKVGDKV LLPEYGGTKV VLDDKDYFLF RDGDILGKYV D | Storage : Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.