Active Recombinant Human FGFR2 protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.2-1 μg/mL. | Molecular Mass : The recombinant human FGFR2 consists of 367 amino acids after removal of the signal peptide and has a calculated molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFGFR2 is approximately 65-75 kDa due to glycosylation. | Protein Length : Met1-Glu377 | Endotoxin : | Purity : > 97 % as determined by SDS-PAGE | Storage : Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.