Product Name :
Rabbit anti-MYOGENIN Polyclonal Antibody(N-term)
Synonym :
Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; BHLHC3; MYF4
Host :
Rabbit
Species Reactivity:
Human, Mouse
Specificity :
This MYOGENIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYOGENIN.
Predicted Reactivity:
Applications :
WB~~1:1000
Immunogen:
Concentration :
Purification :
Clonality:
Polyclonal Antibody
Storage Temp.:
Maintain refrigerated at 2-8 ° C for up to 2 weeksFor long time storage store at -20 ° C in small aliquots to prevent free that cycles
Research areas :
Signal Transduction
Background :
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
UniProt :
P15173
Additional information:
Product Details FAQ Citations(3) Video Pictures Documents |Overview |Description Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |Synonym Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; BHLHC3; MYF4 |Host Rabbit |Specificity This MYOGENIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYOGENIN. |Species Reactivity Human, Mouse |Applications WB~~1:1000 |Properties |Clonality Polyclonal Antibody |Isotype Rabbit Ig |Storage Temp. Maintain refrigerated at 2-8 ° C for up to 2 weeksFor long time storage store at -20 ° C in small aliquots to prevent free that cycles |Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |Research areas Signal Transduction |Target |Background Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity). |Cellular localization Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin- remodeling and transcription initiation in developing embryos |UniProt P15173 |References |References Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010)Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)Ramamoorthy, S., et al. Am. J. Physiol. Endocrinol. Metab. 297 (2), E392-E401 (2009) :Nanni, P., et al. Mol. Cancer Ther. 8(4):754-761(2009)Gong, C., et al. Genes Dev. 23(1):54-66(2009) |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-MYOGENIN Polyclonal Antibody(N-term)
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
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