Active Recombinant Human IGF2 Protein, Fc & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : Fc & Avi | Form : Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose. | Bio-activity : Measured by its binding ability in a functional ELISA against immobilized Human IGFBP-3, His Tag (0.5 μg/well). The linear range is 0.008-0.1 μg/mL. | Molecular Mass : This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc fragment. The protein has a calculated MW of 35.4 kDa. The protein migrates as 40 kDa on a SDS-PAGE gel under reducing condition and 66 kDa under non-reducing condition. | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.