Sequence described in the E26 27 avian erythroblastosis virus (E26 Transformation-specific Sequence). Studies in mutant mice have demonstrated that ETS-1 knockout animals have a lower number of glomeruli, 28 and among the existing glomeruli, a higher number are immature, highlighting the 29 31 important role of ETS-1 in the regulation of normal kidney development. ?The transcriptional PD0325901 web activity of ETS-1 is modulated through post-translational modifications. 32 Phosphorylation of threonine-38 increases the transcriptional activity of ETS-1 while calmodulin-dependent kinase II inhibits DNA binding through serine phosphorylation of 33 ETS-1 inhibitory domains. ETS-1 is also regulated through nuclear transport via specific nuclear localization sequences that facilitate the movement of ETS-1 from the cytoplasm 34 into the nucleus. The renal expression of ETS-1 is increased in a variety of models of renal injury. The antiThy1 model of glomerulonephritis is associated with a 4-fold increase in ETS-1 expression predominantly in the glomerular mesangium and at a lesser degree in podocytes and the 35 glomerular endothelium. In rats with antiglomerular basement nduced glomerulonephritis, there is also increased upregulation of ETS-1 in the glomeruli and in the 36 interstitium, and in an ischemic model of acute renal failure, the tubular expression of ETS-1 is increased and associated with augmented expression of cyclin D, suggesting a role 37 for ETS-1 in the control of tubular regeneration in acute kidney injury. In previous studies, we also demonstrated that Ang II increases the cortical expression of ETS-1 in SpragueDawley rats and that knockdown of ETS-1 reduces Ang II-stimulated fibronectin production 38 in rat mesangial cells. In other studies, we demonstrated that Ang II increases the glomerular expression of ETS-1 in mice and that blockade of ETS-1 using a specific DN 13 reduces proteinuria, inflammation, and fibrosis induced by Ang II. Importantly, in these studies, ETS-1 blockade did not reduce blood pressure, suggesting that hemodynamic effects did not mediate the beneficial actions of ETS-1 blockade. In the present studies, we have demonstrated increased glomerular expression of ETS-1 in hypertensive Dahl/Rapp salt-sensitive rats, a paradigm of salt-sensitive hypertension in humans. We demonstrated that hypertensive Dahl/Rapp salt-sensitive rats have a significant increase in the expression of the GSK343 site phosphorylated (T38) form of ETS-1 without significant changes in the expression of total ETS-1. Although we did not observe changes in the expression of total ETS-1, we cannot rule out that changes in the expression of total ETS-Hypertension. Author manuscript; available in PMC 2016 June 08.Feng et al.Pagemay occur at other time points. Using colocalization methods, we demonstrated that the expression of ETS-1 is mostly glomerular and predominantly expressed in the glomerular epithelium and to a lesser degree in the glomerular endothelium. Several studies have demonstrated that DS rats when fed a high-salt diet and made hypertensive have increased local activation of the RAS, characterized by sustained levels of 14 Ang II, increased levels of angiotensinogen, and increased expression of the AT1 receptor. In addition, as others and we have shown, salt-sensitive hypertension is associated with 2 reduced nitric oxide bioavailability and increased reactive oxygen species production. ,39,40 In support of the role for increased RAS activation in s.Sequence described in the E26 27 avian erythroblastosis virus (E26 Transformation-specific Sequence). Studies in mutant mice have demonstrated that ETS-1 knockout animals have a lower number of glomeruli, 28 and among the existing glomeruli, a higher number are immature, highlighting the 29 31 important role of ETS-1 in the regulation of normal kidney development. ?The transcriptional activity of ETS-1 is modulated through post-translational modifications. 32 Phosphorylation of threonine-38 increases the transcriptional activity of ETS-1 while calmodulin-dependent kinase II inhibits DNA binding through serine phosphorylation of 33 ETS-1 inhibitory domains. ETS-1 is also regulated through nuclear transport via specific nuclear localization sequences that facilitate the movement of ETS-1 from the cytoplasm 34 into the nucleus. The renal expression of ETS-1 is increased in a variety of models of renal injury. The antiThy1 model of glomerulonephritis is associated with a 4-fold increase in ETS-1 expression predominantly in the glomerular mesangium and at a lesser degree in podocytes and the 35 glomerular endothelium. In rats with antiglomerular basement nduced glomerulonephritis, there is also increased upregulation of ETS-1 in the glomeruli and in the 36 interstitium, and in an ischemic model of acute renal failure, the tubular expression of ETS-1 is increased and associated with augmented expression of cyclin D, suggesting a role 37 for ETS-1 in the control of tubular regeneration in acute kidney injury. In previous studies, we also demonstrated that Ang II increases the cortical expression of ETS-1 in SpragueDawley rats and that knockdown of ETS-1 reduces Ang II-stimulated fibronectin production 38 in rat mesangial cells. In other studies, we demonstrated that Ang II increases the glomerular expression of ETS-1 in mice and that blockade of ETS-1 using a specific DN 13 reduces proteinuria, inflammation, and fibrosis induced by Ang II. Importantly, in these studies, ETS-1 blockade did not reduce blood pressure, suggesting that hemodynamic effects did not mediate the beneficial actions of ETS-1 blockade. In the present studies, we have demonstrated increased glomerular expression of ETS-1 in hypertensive Dahl/Rapp salt-sensitive rats, a paradigm of salt-sensitive hypertension in humans. We demonstrated that hypertensive Dahl/Rapp salt-sensitive rats have a significant increase in the expression of the phosphorylated (T38) form of ETS-1 without significant changes in the expression of total ETS-1. Although we did not observe changes in the expression of total ETS-1, we cannot rule out that changes in the expression of total ETS-Hypertension. Author manuscript; available in PMC 2016 June 08.Feng et al.Pagemay occur at other time points. Using colocalization methods, we demonstrated that the expression of ETS-1 is mostly glomerular and predominantly expressed in the glomerular epithelium and to a lesser degree in the glomerular endothelium. Several studies have demonstrated that DS rats when fed a high-salt diet and made hypertensive have increased local activation of the RAS, characterized by sustained levels of 14 Ang II, increased levels of angiotensinogen, and increased expression of the AT1 receptor. In addition, as others and we have shown, salt-sensitive hypertension is associated with 2 reduced nitric oxide bioavailability and increased reactive oxygen species production. ,39,40 In support of the role for increased RAS activation in s.
