nrelated to Bt-resistance coding genes (FigureBt-resistance related gene was less than one hundred kb up- or downstream from the HDAC2 Accession lncRNA (Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 (this lncRNA was downregulated) (Figure 4B), along with the serine AMPK review protease 0.646 kb from lncRNA LOC110382674 (this lncRNA was discovered only within the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was downregulated, along with the lncRNA presented in Figure 4E was identified only inside the susceptible strain.Insects 2022, 13,(Figure 4E). Each proximal Bt-resistance related gene was much less than one hundred kb up- or downstream in the lncRNA (Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 downstream was downregulated) resistance related gene was less than one hundred kb up- or (this lncRNAfrom the lncRNA (Fig(Figure 4B), along with the serine protease 0.646 from lncRNA LOC11350610 (this lncRNA was ure 4A ). The proximal CYP was 7.868 kbkb from lncRNA LOC110382674 (this lncRNA of 18 was discovered only within the resistant proximal ABC transporter 50.672 kb in Figure9 4D upregulated) (Figure 4A), the strain) (Figure 4C). The lncRNA presentedfrom lncRNA was downregulated,lncRNA was downregulated)in Figure4B), plus the serine protease and the lncRNA presented (Figure 4E was located only within the LOC110369725 (this susceptible strain. 0.646 kb from lncRNA LOC110382674 (this lncRNA was discovered only within the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was downregulated, and also the lncRNA presented in Figure 4E was discovered only in the susceptible strain.Figure 3. Workflow for identifying statistically differentiated lncRNAs coding genes in toto and Figure 3. Workflow for identifying statistically in Bt-resistance lncRNAs proximal in toto and these Figure with functions known to have a function differentiated lncRNAsare coding genesstatistically those 3. Workflow for identifying statistically differentiated that coding genes to in toto and with with functions known to possess ain Bt-resistance which are proximal size to statistically differendifferentiated identified to possess a function role in Bt-resistance which might be proximal in the scaffolds, even those functions lncRNAs. Proximity measurements had been restricted by theto statistically differentiated lncRNAs. Proximity measurements million base by thecis as well as the scaffolds,scaffolds, although though proximity is defined as 1 were restricted pairs by of size from despite the fact that proximity tiated lncRNAs. Proximity measurements were limitedsize the trans with the the lncRNA. For each proximal as 1 million and lncRNA, a BLASTn alignment was lncRNA. For each and every coding gene and proximity is defined as base pairsbase pairs cis and trans lncRNA. also each proximalproximal coding is defined coding gene 1 million cis and trans from the in the For performed to assess possible pseudogenes. gene and lncRNA, a alignment was also conducted to assess possible possible pseudogenes. lncRNA, a BLASTn BLASTn alignment was also conducted to assess pseudogenes.(A)Figure four. Cont.Insects 2022, 13, 12 Insects 2022, 1, x10 of 18 ten of(B)(C)Figure 4. Cont.Insects 2022, 13, 12 Insects 2022, 1, x11 of 18 11 of(D)(E)Figure four. Genomic scaffold for lncRNAs and identification of proximal protein-coding genes. The Figure 4. Genomic scaffold for lncRNAs and id
