hich is equivalent to that of other Insects 2021, 12, x FOR PEER Assessment 8 of 19 species studied here (Supplementary Supplies, Table S2), indicating that its sequences were normal transcripts. Therefore, the 25 fig wasp transcriptomes have been all well-assembled and fairly complete.Figure 1. Functional characters of 25 fig wasps : (a) the total quantity of proteins annotated for 25 fig wasps as well as the completeness on the transcriptomes identified for them by BUSCO; (b) proportions of genes annotated by Nr, COG, KAAS, Figure 1. Functional characters wasps. GO, and all datasets for the 25 figof 25 fig wasps : (a) the total quantity of proteins annotated for 25 fig wasps plus the completeness in the transcriptomes identified for them by BUSCO; (b) proportions of genes annotated by Nr, COG, KAAS, GO, and all datasets3.two. the 25 fig wasps. for Unigenes Annotated against Public DatabasesBlasting of BLASTP or HMM against protein databases (Nr, Swiss-Prot, KOG, eggNOG, three.two. Unigenes and GO) against Public 7859 to 52,340 unigenes (median: 10,437) have been sucPfam, KEEG,Annotated revealed that Databases Blasting of BLASTP or HMM against protein databases (Nr, Swiss-Prot, KOG, eggNOG, Pfam, KEEG, and GO) revealed that 7859 to 52,340 unigenes (median: ten,437) had been effectively annotated for 25 fig wasps (Figure 1; Supplementary Components, Table S2), as well as the proportions of unigenes annotated ranged from 75.99 to 89.53 (median: 84. 70 ). The proportions annotated against Nr and eggNOG had been fairly high, withInsects 2021, 12,eight ofcessfully annotated for 25 fig wasps (Figure 1; Supplementary Components, Table S2), and also the proportions of unigenes annotated ranged from 75.99 to 89.53 (median: 84. 70 ). The proportions annotated against Nr and eggNOG had been relatively high, with Nr from 71.45 to 85.89 (median: 78.97 ) and eggNOG from 61.03 to 82.72 (median: 76.16 ). The proportion annotated against the PFAM was the third highest (from 61.99 to 74.83 ; median: 69.14 ). The proportions annotated against other databases are given in Table S2. In total, 17,952 and 21,285 OCGs might be annotated by GO and KEGG, respectively, for downstream enrichment evaluation. three.three. Phylogenetic Relationships The phylogenetic tree generated from 625 single-copy homologous genes was further integrated into one particular final tree with all branches supported by one hundred bootstrap support (Figure 2b). All clades of dioecious fig wasps had been ALK1 review monophyletic, while the monoecious fig wasps were polyphyletic, with Eupristina altissima and Platyscapa sp.-F. rumphii branching first Insects 2021, 12, x FOR PEER Evaluation and also the other two Platyscapa species inside a different clade. Here, we considerof 19 9 them as 1 genus clade Eu/Pl.Figure 2. Phylogenetic relationships with gene-family clusters of 25 fig wasps and 7 other insect species: (a) comparison Figure 2. Phylogenetic relationships with gene-family clusters of 25 fig wasps and 7 other insect species: (a) comparison of your numbers of expanded (dark line) and contracted (light line) gene families amongst wasps (nodes represent the of your numbers of expanded (dark line) and contracted (light line) gene families among 25 fig 25 fig wasps (nodes represent number of gene Macrolide Accession households); (b) the resultant phylogenetic relationships, like divergence time plus the variety of exthe variety of gene households); (b) the resultantbranches and ideas (massive numbers of contracted gene families arethe quantity of phylogenetic relationships, such as divergence time and shown in panded/
