Active Recombinant Human CDH1 Protein, Fc Chimera

Specification :
| Source : Mammalian cells | Species : Human | Tag : Fc | Predicted N Terminal : Asp155 | Form : Lyophilized from a 0.2 µm filtered solution in Tris-Citrate, NaCl and CaCl2. | Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >60% cells will adhere after 90 minutes at 37 °C.Optimal concentration depends on cell type as well as the application or research objectives. | Molecular Mass : Recombinant Human CDH1, Fc Chimera has a calculated MW of 87.7 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions. | Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. | Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). | Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.