Active Recombinant Human CEACAM1 protein, His-tagged

Specification :
| Source : Mammalian cells | Species : Human | Tag : His | Predicted N Terminal : Gln35 | Form : Lyophilized from a 0.2 μm filtered solution in PBS. | Bio-activity : Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544. rhCEACAM-1 at concentrations higher than 1 µg/mL reduces anti-CD3 (immobilized at 10 µg/mL) induced IL-2 production by 60-70%.The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is typically 0.05-0.2 µg/mL. | Molecular Mass : Predicted Molecular Mass: 44.6 kDa;SDS-PAGE: 100-130 kDa, reducing conditions. | Endotoxin : | Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. | Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 100 μg/mL in sterile PBS.