Recombinant Human CD80 protein, Avi & His-tagged, Biotinylated

Specification :
| Description : B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. | Source : HEK293 | Species : Human | Tag : Avi & His | Predicted N Terminal : Val 35 | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. | Molecular Mass : The protein has a calculated MW of 26.5 kDa. The protein migrates as 44-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein length : Val 35 – Asn 242 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.