Product: SM1872PS5-Methyl Cytosine / 5-MeC antibody
Quantity: 50 µg
Synonyms: 5-MeCyd, 5-Methyl Cytidine, 5-Methylcytidine, 5MeC
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 1395347-24-6 Product: ISA-2011B
Shipping to: Worldwide
Immunogen: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0Ag 14 myeloma cell line
Application: Flow Cytometry: Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in Ref.4 (Giraldo, A. M. et al.)Immunoblotting.Immunofluorescence.Immunohistochemistry on Frozen and Paraffin Embedded Sections:
Background: Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when Cytosine is attached to a Ribose ring (also known as a Ribofuranose) via a beta-N1-glycosidic bond.
Concentration: 1.0 mg/ml
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 10 mM PBS, pH 7.4
Preservatives: 0.01% Thimerosal
State: Liquid purified IgG fractionPurified
Specifictiy: This antibody recognises the modified base 5-Methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression.Clone 33D3 has been developed to discriminate
