Recombinant Human CDH1 protein, His & S-tagged

Specification :
| Description : This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. | Source : E. coli | Species : Human | Tag : His & S | Form : Freeze-dried powder | Molecular Mass : Predicted Molecular Mass: 33.1kDa | Protein length : Pro373~Pro621 (Accession # P12830) | Endotoxin : | Purity : > 95% | Characteristic : The isoelectric point is 4.5. | Applications : SDS-PAGE; WB; ELISA; IP. | Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. | Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. | Storage buffer : PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. | Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4