Recombinant Human CDK4 protein, His/T7-tagged

Specification :
| Description : Cyclin-Dependent Kinase 4 (CDK4) is a member of the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK4 is a component of Cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. These complexes are major integrators of various mitogenenic and antimitogenic signals. It is shown that CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb). Defects in CDK4 are a cause of susceptibility to cutaneous Malignant Melanoma Type 3. | Source : E. coli | Species : Human | Tag : His/T7 | Form : Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. | Protein length : Met1-Glu303 | AA Sequence : MGSSHHHHHHSSGLVPRGSHMASMT GGQQMGRGSMATSRYEPVAEIGVGA YGTVYKARDPHSGHF VALKSVRVPNGGGGGGGLPISTVRE VALLRRLEAFEHPNVVRLMDVCATS RTDREIKVTLVFEHV DQDLRTYLDKAPPPGLPAETIKDLM RQFLRGLDFLHANCIVHRDLKPENI LVTSGGTVKLADFGL ARIYSYQMALTPVVVTLWYRAPEVL LQSTYATPVDMWSVGCIFAEMFRRK PLFCGNSEADQLGKI FDLIGLPPEDDWPRDVSLPRGAFPP RGPRPVQSVVPEMEESGAQLLLEML TFNPHKRISAFRALQ HSYLHKDEGNPE | Endotoxin : Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. | Purity : Greater than 95% as determined by reducing SDS-PAGE. | Storage : Lyophilized protein should be stored at Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.Aliquots of reconstituted samples are stable at | Reconstitution : Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | Shipping : The product is shipped at ambient temperature.