Active Recombinant Human CTLA4, Fc-tagged

Specification :
| Description : CD152 [CTLA-4] and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28. | Source : CHO Cells | Species : Human | Tag : Fc | Form : Lyophilized from 0.2μm-filtered solution in PBS. | Bio-activity : Binds both CD80 (B7-1) and CD86 (B7-2) with high affinity and inhibits CD28 signaling competitively. Kills the target cell completely. | Purity : ≥98% (SDS-PAGE) | Stability : Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. | Storage : Short Term Storage: +4°C; Long Term Storage: -20°C. Avoid freeze/thaw cycles.