Name :
Active Recombinant Human CTLA4 protein, Twin Strep-tagged
Specification :
| Source : HEK293 | Species : Human | Tag : Twin Strep | Predicted N Terminal : Ala 37 | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD80 /B7-1 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-2 / CD86 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL. | Molecular Mass : Has a calculated MW of 16.5 kDa. DTT-reduced Protein migrates as 21-26 kDa in SDS-PAGE due to glycosylation. | Protein length : Ala 37 – Phe 162 | Endotoxin : Less than 1.0 EU per μg of the rh CTLA4 /CD152,twin strep tag by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
Gene Information :
| Gene Name : CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ] | Official Symbol : CTLA4 | Synonyms : CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3; | Gene ID : 1493 | mRNA Refseq : NM_005214 | Protein Refseq : NP_005205 | MIM : 123890 | UniProt ID : P16410 | Chromosome Location : 2q33 | Pathway : Adaptive Immune System, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CTLA4 inhibitory signaling, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; | Function : protein binding;
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