Product: DP35146xHistidine Epitope Tag (HHHHHH) antibody
Quantity: 50 µg
Synonyms:
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype:
CAS NO: 1380341-99-0 Product: GAL-021
Shipping to: Worldwide
Immunogen: Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.
Application: Western blot (0.5-1 µg/ml): The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli.
Background: In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag”
Concentration:
Storage: The lyophilized IgG is stable at 2-8°C for one month from despatch and for one year when kept at -20°C.The reconstituted antibody can be stored at 2-8°C for one month or at -20°C for one year.Avoid repeated freezing and thawing.
Buffer System: PBS, pH 7.4, without preservatives.
Preservatives:
State: Lyophilized purified IgG fraction.Aff – Purified
Specifictiy: Western analysis with several His-tagged proteins either expressed in insect cells or E.coli showed that the antibody recognizes all tested proteins fused to a C-terminal but not to a N-terminal His-tag.The antibody might be a very good tool to test super
