Active Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, mFc-tagged, low endotoxin

Specification :
| Source : HEK293 | Species : Human | Tag : mFc | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag with a linear range of 0.1-5 μg/mL. | Molecular Mass : The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Asn 19 – Pro 152 | Endotoxin : Less than 0.1 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.Please avoid repeated freeze-thaw cycles.This product is stable after storage at:-20 centigrade to -70 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.