Active Recombinant Human CSF1R protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human CSF1R at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1, The EC50 of biotinylated human CSF-1 is 8 ng/mL. | Molecular Mass : The recombinant human M-CSFR contains 504 amino acids after the removal of signal peptide with a calculated molecular mass of 56 kDa. As a result of glycosylation, it migrates as an approximately 85-95 kDa protein in SDS-PAGE under reducing conditions. | Protein Length : Met1-Glu512 | Endotoxin : | Purity : > 98 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.