Active GMP Recombinant Human FN1 Protein

Specification :
| Description : Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result. | Source : E. coli | Species : Human | Form : Lyophilized. | Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 1×10E5 cells/well areadded to Fibronectin-coated plates (7-13 ng/mL and 100μL/well), approximately 50%-80% will adhere specifically after 30 minutes at 37°C. | AA Sequence : MPTDLRFTNIGPDTMRVTWAPPPSI DLTNFLVRYSPVKNEEDVAELSISP SDNAVVLTNLLPGTE YVVSVSSVYEQHESTPLRGRQKTGL DSPTGIDFSDITANSFTVHWIAPRA TITGYRIRHHPEHFS GRPREDRVPHSRNSITLTNLTPGTE YVVSIVALNGREESPLLIGQQSTVS DVPRDLEVVAATPTS LLISWDAPAVTVRYYRITYGETGGN SPVQEFTVPGSKSTATISGLKPGVD YTITVYAVTGRGDSP ASSKPISINYRTEIDKPSMAIPAPT DLKFTQVTPTSLSAQWTPPNVQLTG YRVRVTPKEKTGPMK EINLAPDSSSVVVSGLMVATKYEVS VYALKDTLTSRPAQGVVTTLENVSP PRRARVTDATETTIT ISWRTKTETITGFQVDAVPANGQTP IQRTIKPDVRSYTITGLQPGTDYKI YLYTLNDNARSSPVV IDASTAIDAPSNLRFLATTPNSLLV SWQPPRARITGYIIKYEKPGSPPRE VVPRPRPGVTEATIT GLEPGTEYTIYVIALKNNQKSEPLI GRKKTDELPQLVTLPHPNLHGPEIL DVPST | Residual Host Cell DNA Content : | Residual Host Cell Protein Content : | Endotoxin : | Purity : Greater than 95% as determined by reducing SDS-PAGE. | Storage : Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | Reconstitution : Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration >100μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. | Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.