Active Recombinant Human DPP4 protein

Specification :
| Source : HEK293 | Species : Human | Tag : N/A | Form : Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant. | Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >11000 pmol/min/µg. | Molecular Mass : This protein carries no “tag”The protein has a calculated MW of 85.5 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Asn29-Pro766 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. | Publication : Comparison of the susceptibility of porcine and human dipeptidyl-peptidase IV to inhibition by protein-derived peptides (2015) Peptide Array on Cellulose Support-A Screening Tool to Identify Peptides with Dipeptidyl-Peptidase IV Inhibitory Activity within the Sequence of α-Lactalbumin. (2014)