Active Recombinant Human ENPP3 protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Measured by its ability to hydrolyze the Pyrophosphate (PPi) from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 100 pmol/min/μg. | Molecular Mass : This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 98.6 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Leu48-Ile875 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.