Active Recombinant Human DGKA protein, GST-tagged

Specification :
| Description : Diacylglycerol kinase alpha (DAG kinase alpha) is a member of the eukaryotic diacylglycerol kinase family. Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resyn-thesis of phosphatidylinositols and attenuating protein kinase C activity. Involved in hepatocellular carcinoma progression through regulation of the Ras/Raf/MEK/ERK pathway. The DGKα/atypical PKC/β1 integrin signaling pathway is required for matrix invasion of breast carcinoma cells. | Source : Insect cells | Species : Human | Tag : GST | Form : 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. | Bio-activity : 3000 nmol/min/mg | Molecular Mass : 105 kDa | Purity : >90% | Applications : Kinase Assay | Stability : 1 year at -70 centigrade from the date of shipment. | Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. | Concentration : 0.1μg/μl