Recombinant Human DGKE protein, His-tagged

Specification :
| Product overview : Recombinant Human DGKE (P52429) aa. (Leu243~Gly502) fused with His tag was produced in E. coli cells. | Description : Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. | Source : E. coli | Species : Human | Tag : His | Form : Freeze-dried powder | Molecular Mass : Predicted Molecular Mass: 32.5kDa; Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. | Protein length : Leu243~Gly502 | Purity : >95% | Characteristic : Isoelectric point is 7.7. | Applications : Cell culture; Activity Assays; In vivo assays. | Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. | Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. | Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. | Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex