Active Recombinant Human EGFR protein, Fc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : Fc | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant. | Bio-activity : Immobilized Human EGFRvIII, Fc Tag at 2 μg/mL (100 μL/well) can bind Anti-EGFRvIII Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL. | Molecular Mass : This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 65.1 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Leu25-Ser378 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.