Active Recombinant Human EGFR protein, Fc-tagged, APC labeled

Specification :
| Source : HEK293 | Species : Human | Tag : Fc | Form : Lyophilized from sterile 50 mM Tris, pH 8.0.Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml. | Molecular Mass : The recombinant human EGFR/Fc chimera is a homodimeric protein. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110-140 kDa protein in SDS-PAGE under reducing conditions. | Protein Length : Met 1-Ser 645 | Endotoxin : | Purity : > 97 % as determined by SDS-PAGE. >85% as determined by SEC-HPLC | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.