Active Recombinant Human ERBB2 Protein, Fc/His-tagged, Alexa Fluor® 647 conjugated

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. | Source : Mouse myeloma cell line | Species : Human | Tag : Fc/His | Form : Disulfide-linked homodimerLabeled with Alexa Fluor® 467Excitation Wavelength: 650 nmEmission Wavelength: 668 nm | Bio-activity : Measured by flow cytometry for its ability to bind anti-Human ErbB2/Her2 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human ErbB2/Her2 Fc Chimera Alexa Fluor® 647 that produces 50% of the binding response is 0.50-10.0 ng/mL | Molecular Mass : 97 kDa | N-terminal Sequence Analysis : Thr23 | Endotoxin : | Purity : >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. | Storage : Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after opening.3 months, -20 to -70 centigrade under sterile conditions after opening. | Storage Buffer : Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Shipping : The product is shipped with dry ice or equivalent.