Recombinant Human EIF2AK2 protein, His-tagged

Specification :
| Product overview : Recombinant Human EIF2AK2 (P19525) aa. (Ser224~Ile502) fused with His tag was produced in E. coli cells. | Description : The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. | Source : E. coli | Species : Human | Tag : His | Form : Freeze-dried powder | Molecular Mass : Predicted Molecular Mass: 35.8kDa; Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions. | Protein length : Ser224~Ile502 | Purity : >80% | Characteristic : Isoelectric point is 8.9. | Applications : Cell culture; Activity Assays. | Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. | Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. | Storage buffer : 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300. | Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex