Active Recombinant Human EphA2 protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 His at 2 μg/ml (100 μl/well) can bind Human Ephrin-A1 His & hFc, the EC50 of Human Ephrin-A1 His & hFc is 4.0-20.0 ng/mL. | Molecular Mass : The recombinant human EphA2 consists of 522 amino acids and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions. | Protein Length : Met1-Asn534 | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.