Active Recombinant Human EPHA3 protein(Met1-Gln541), His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : C-His | Protein length : Met1-Gln541 | Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized human EphA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6.2-14.6 ng/ml. | Molecular Mass : The recombinant human EphA3 consists of 532 amino acids and predicts a molecular mass of 60.3 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions. | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.