Active Recombinant Human ERBB2 protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human Her2 (S310Y), His Tag at 1 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.1-3 ng/mL.Immobilized Human Her2 (S310Y), His Tag at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.2-12.5 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 71.3 kDa. The protein migrates as 80-100 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Thr23-Thr652 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.