Active Recombinant Human EPHB1 Protein, GST-tagged

Specification :
| Description : Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008] | Source : Insect cells | Species : Human | Tag : GST | Form : Liquid | Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.Substrate: Blk/Lyntide.ATP: 100 μM. | Molecular Mass : 73 kDa | Purity : 68 % by SDS-PAGE/CBB staining. | Applications : Functional StudySDS-PAGE | Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. | Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)