Recombinant Human ERBB2 Protein (ECD), His-tagged(C-ter)

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] | Source : HEK293 | Species : Human | Tag : His(C-ter) | Form : Powder | Protein Length : Thr23-Thr652 | Endotoxin : Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test. | Purity : > 95% (by SDS-PAGE) | Applications : Binding, SDS-PAGE | Notes : For laboratory research only, not for drug, diagnostic or other use. | Storage : Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. | Storage Buffer : PBS (pH 7.4) | Reconstitution : Reconstitute to a concentration of 0.1-0.5 mg/ml in sterile distilled water.